|本期目录/Table of Contents|

 Electric Impedance Spectroscopy for On-Chip Analysis of Human
Trabecular Meshwork Endothelium
(PDF)

《纳米技术与精密工程》[ISSN:1672-6030/CN:12-1351/O3]

期数:
2013年3期
页码:
211-216
栏目:
微机电系统
出版日期:
2013-05-15

文章信息/Info

Title:
 阻抗谱芯片用于人眼小梁网细胞分析
作者:
 Gu Wenwen1 Zhao Yi2
 (1. College of Engineering and Technology, Southwest University, Chongqing 400715, China;
2. Department of Biomedical Engineering, The Ohio State University, Columbus 43210, USA)
Author(s):
 顾雯雯1 赵 毅2
 (1.西南大学工程技术学院,重庆400715; 2.美国俄亥俄州立大学生物医学工程系,哥伦布 43210)
关键词:
electric impedance spectroscopy interdigitated microelectrode human trabecular meshwork primary open angle glaucoma
Keywords:
阻抗谱 叉指式电极 人眼小梁网 开角型青光眼
分类号:
TP212.3
DOI:
-
文献标识码:
A
摘要:
 The in vitro cultured human trabecular meshwork (TM) was investigated using electric impedance spectroscopy (EIS) in real time. TM is a critical determinant of intraocular pressure (IOP), the increase of which is the main cause of glaucoma. An array of interdigitated microelectrodes (IMEs) was fabricated for electric impedance sensing.An equivalent electric circuit model was proposed to study the cellular activities on the IMEs, showing that the impedance of extracellular matrix (ECM) and the cell coverage ratio have distinct signatures on the spectrum. The utility of the EIS was demonstrated by treating TM cells during their proliferation with dexamethasone (DEX). The impedance measurement at 40 kHz showed that a higher DEX concentration can induce more severe inhibition of cell proliferation. The measurement was validated by AlamarBlue assay. The measurement provided a reasonable explanation on glucocorticoid induced glaucoma, where the glucocorticoid alters the metabolic and physiological functions of TM cells by inhibiting normal cell growth and proliferation.
Abstract:
 采用阻抗谱分析法对体外培养人眼小梁网细胞进行实时在线检测.人眼眼压过高将导致青光眼病变,而小梁网对于眼压高低至关重要.制备了叉指式阵列微电极作为阻抗传感器敏感单元.提出了分析小梁网细胞生物行为特征的等效电路模型,通过仿真分析表明该电路模型可在不同频段区分人眼小梁网细胞外基质和细胞繁殖率等阻抗参数值.采用叉指式阻抗谱芯片对经地塞米松药物处理的小梁网细胞进行了实时在线监测,结果表明40 kHz时高浓度地塞米松对小梁网细胞增殖具有较大的抑制作用,该结论与AlamarBlue试剂法得到的结论一致.阻抗测试结果为糖皮质激素诱导的青光眼提供了一种合理的解释,认为该病变与正常细胞增殖率异常有关.

参考文献/References

备注/Memo

备注/Memo:
收稿日期: 2013-02-03.
基金项目: 中央高校基本科研业务费专项资金资助项目(XDJK2013C011);西南大学博士基金资助项目(SWU112050).
作者简介: 顾雯雯(1983— ),女,讲师,博士.
通讯作者: 顾雯雯,guww1983@163.com.
更新日期/Last Update: 2013-05-22