|本期目录/Table of Contents|

新型网状通道微流控细胞培养芯片设计与制作(PDF)

《纳米技术与精密工程》[ISSN:1672-6030/CN:12-1351/O3]

期数:
2017年4期
页码:
273-279
栏目:
微机电系统
出版日期:
2017-07-15

文章信息/Info

Title:
Design and Fabrication of a Novel Microfluidic Cell Culture Chip for Mesh Channel
作者:
刘海侠 刘冲 魏娟 刘涛 李经民
大连理工大学微纳米技术及系统辽宁省重点实验室, 大连116024
Author(s):
Liu Haixia Liu Chong Wei Juan Liu Tao Li Jingmin
Key Laboratory for Micro/Nano Technology and System of Liaoning Province, Dalian University of Technology, Dalian 116024, China
关键词:
微流控 细胞培养 稳定 粒子图像测速
Keywords:
microfluidic cell culture stability particle image velocimetry
分类号:
R318.6
DOI:
10.13494/j.npe.20160119
文献标识码:
A
摘要:
微流控芯片能为细胞的体外培养提供良好的生长微环境.设计制作了一系列具有网状微通道网络的细胞培养微流控芯片,为细胞体外培养提供了稳定的流体环境.该芯片由网状微通道网络和处于网状通道节点处的培养腔室组成.芯片整体结构呈对称分布,外部流体在驱动力的作用下进入微通道网络,经过多条微通道进入培养池.培养池周围微通道具有“多入多出”的特点,在很大程度上提高了培养池内流场的稳定性.同时研究了芯片微通道网络的网状程度、培养腔数量对培养池内流场的影响.结果表明:处于网状微通道网络上的培养池内流场均一稳定,能为细胞体外培养提供近体微环境.
Abstract:
Microfluidic chips can provide a good microenvironment for cell culture in vitro. We designed and fabricated a series of microfluidic microarray chips with a network of reticular channels to provide a stable fluid environment for cell culture in vitro. The chip consists of a mesh microchannel network and a culture chamber at the node of the mesh channel. The overall structure of the chip is symmetrically distributed, with external fluid flowing into the microchannel network under the driving force, and into the culture chamber via a number of microchannels. The microchannel around the culture chamber has the characteristics of “multi-in and multi-out”, improving the stability of the flow field in the culture pool to a large extent. At the same time, we studied the network degree of the microchannel network and the influence of the number of culture chambers on the flow field in the culture chamber. Results indicate that the flow field in the reticular microchannel network is stable and can provide the near-body microenvironment for cell culture in vitro.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期: 2017-03-08. 基金项目: 国家自然科学基金资助项目(51375076). 作者简介: 刘海侠(1989—),女,硕士研究生. 通讯作者: 李经民,副教授jingminl@dlut.edu.cn.
更新日期/Last Update: 2017-12-20