|本期目录/Table of Contents|

玻璃基底上的蛋白质微接触压印图型化

《纳米技术与精密工程》[ISSN:1672-6030/CN:12-1351/O3]

期数:
2009年6期
页码:
0
栏目:
精密加工
出版日期:
2009-11-15

文章信息/Info

Title:
Using Microcontact Printing to Pattern the Proteins on Glass Surface
作者:
叶芳1苑伟政1谢丽2
1.西北工业大学陕西省微/纳米系统重点实验室,西安,710072;2.西北工业大学生命科学院,西安,710072
Author(s):
YE Fang YUAN Wei-zheng XIE Li
关键词:
微接触印刷 胶原Ⅰ型蛋白 氧等离子体 聚二甲基硅氧烷 亲水性
Keywords:
microcontact printing Col- Ⅰprotein oxygen plasma polydimethylsiloxane hydrophilicity
分类号:
-
DOI:
-
文献标识码:
-
摘要:
研究了一种将胶原Ⅰ型蛋白通过微接触压印技术图型化于玻璃基底表面的方法.采用标准光刻工艺制备印章母版,并运用反应离子刻蚀设备对印章表面进行氧等离子体处理,以期改善印章表面亲水性能.将涂敷了胶原Ⅰ型蛋白,并经返潮处理的印章以50 g/cm~2大小的力与玻璃表面接触10 s,得到蛋白质微图型.结果表明,采用反应离子刻蚀技术能显著改善聚二甲基硅氧烷(PDMS)印章表面的亲水性.表面亲水性得到改善的PDMS印章,在经过湿盒返潮后,再进行微接触压印得到的蛋白质微图型其质量得到显著提高.
Abstract:
A method for patterning Col- Ⅰprotein solutions onto glass surfaces with mirco contact printing has been discussed. The stamp master was fabricated with traditional photolithography technique. To improve the hydrophilicity of the polydimethylsiloxane (PDMS) stamps, the surface of the stamps was treated with O_2 plasma. After that, the stamps were coated with an aqueous Col- Ⅰprotein solution, dried in a soft nitrogen air stream and placed in a wet box to return damp for 1 min in order to gain high quality protein pattern. Then the stamps were kept contact with glass substrate for 10 s under the pressure of 50 g/cm~2, which has resulted in better defined Col- Ⅰ micropatterns on the surface. The result shows that the hydrophilicity of the PDMS stamp surface is improved by O_2 plasma. After damping the stamp in a wet box, the micropatterning quality of Col- Ⅰ can be significantly increased.

参考文献/References

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备注/Memo

备注/Memo:
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更新日期/Last Update: 2009-12-20